GSM5036534: Aire C313Y HET WCE rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Aire
Cell type
Cell type Class
Others
Cell type
Thymic epithelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Aire_C313Y_HET_WCE_rep1
strain
NOD X C57BL/6 F1
tissue
mTEChi
age
4-7 weeks
genotype
Aire +/C313Y
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Thymi were removed and enzymatically dissociated with Liberase TH and DNase. mTEChi were sorted and Aire binding sites in the genome were determined by ChIPseq. ChIPseq libraries were prepared for sequencing using a modified ChIPseq protocol (Blecher-Gonen et al., 2013, Bansal et al, 2017)